DNA Test Sample
DNA01 is Plasmid pGem7zf+ (Promega) which is linearized with the SmaI endonuclease. A linear DNA molecules (3000 b. p.) are deposited onto freshly cleaved mica. Molecules are uniformly distributed on the surface with molecule density – 0.5-7 molec./um2 and typical DNA length 1009nm. Recommended humidity for obtaining a good image is 3-5%.
Key Features
Applications:
– getting start in working on AFM with biological samples;
– using like a sample for preparing DNA samples by your own;
– estimation of tip radius of curvature (since the DNA size is equal two nanometres in free condition);
– test samples for Z-resolution of commercial SPMs.
